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The arsS orthologs HH1607 from H. hepaticus ATCC51449 [32], CJ1262 from C. jejuni 4344 [33] and WS1818 from W. succinogenes DSMZ1740 [34] were PCR-amplified from chromosomal DNA of the respective strain using primer pairs HH1607-5/HH1607-3, CJ1262-5/CJ1262-3 and WS1818-5/WS1818-3, respectively.
By contrast, the combined overexpression of argF and argBfbr entailed L-citrulline production and the respective strain was named CIT1.
The asterisk above a bar indicates a significant difference (p < 0.05) between the respective strain and the wild-type strain OH11.
This raises the question whether these dihydropyrimidinase and the hydantoinase activities originate from one enzyme or two different enzymes, a hydantoinase and a dihydropyrimidinase in the respective strain.
Plastic strains and their respective strain gradients produced by nanoindentation have been theoretically interpreted and experimentally measured at shallow indentation depths.
Expressions are provided for the effective moduli tensors of n-phase composites based on the respective strain and electric field concentration tensors.
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Green dotted lines are shown to estimate the average crack widths at the respective strains.
Probes designed for two strains uniquely recognized the respective strains in Q-PCR with a detection limit of 12,5 ng DNA.
The 50%% inhibitory concentration (IC50) was evaluated using different concentrations of cyanobacterial extracts equivalent to 1 µg to 10 mg dry weight of respective strains.
Comparing crack patterns created by the respective strains, the average crack width (1.09 μm) at 50% strain is larger than that (0.72 μm) at 30% strain, and the buckling density is also larger at a higher strain state.
The chloroplasts were transformed such that the psbA site was replaced with genes encoding for GFP or luciferase (Gluc) (Figure 1a), generating the respective strains: CC-5117 psbA::pGFP mt+ and CC-5118 pGluc:pGluc mt+.
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