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We also selected for the, respective, sibling 35S A9 lines, which were used as the proper, single-transgenic control, lines.
Upon apoptotic morphology (cellular shrinkage, blebbing) of an individual cell, the scan area was reduced to include the respective sibling cell.
For instance, the network in Figure 2 is not tree-child, since all the children of v are hybrid, while it is tree-sibling, since the hybrid nodes A and B have respective sibling nodes 1 and 4.
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Gene expression in cell lines from two of these twin pairs were also directly compared against the gene expression profile in cell lines from their respective "normal" sibling.
For this study, we selected autistic male individuals from the distinct subgroup associated with severe language impairment, each of whom had a male sibling who was not affected by autism who served as a control in a paired statistical analysis of gene expression data derived from LCL of the respective siblings.
Values are mean log2 ratio) measures of gene expression between a twin and his respective normal sibling, obtained by DNA microarray analyses with dye reversal replicates * Values in parentheses are mean log2 ratio) measures obtained by triplicate qPCR analyses.
Moreover, when the expression profile of cells from the autistic twin was directly compared against that of his respective normal sibling in a dye-reversal microarray experiment, neurologically relevant genes represented 3 of the top 5 most differentially expressed genes (Table 2).
Interestingly, the mean log2 ratio) of each of these, ASS, CHL1, and FLAP, are higher for the autistic twin than for the more mildly affected twin when each is compared against their respective normal sibling, suggesting a quantitative relationship between differential gene expression (relative to normal individuals) and severity of autistic symptoms, at least for these specific genes.
If the eligible proband had at least one sibling with hypertension, all available full biologic siblings of the index hypertensive, including normotensive siblings, were invited to participate in interviews, physical examinations, and phlebotomy at their respective centers.
Sibling relations.
Sister by Sibling Knits!
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com