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The respective selection marker cassettes were used to derive mini-Tn7 elements that can be used for single-copy insertion of genes into bacterial chromosomes.
After making the respective selection, the system also gives to the users more information concerning the artifact with both visual material (pictures or videos) and additional information in text.
Cells were seeded in their respective selection medium to reach ∼50% confluency and allowed to grow for 2 days.
And fourth, differences between the experimental assay environment and the respective selection regimens led to reduced courtship by ML-evolved males as an artefact.
The latest generations of microarrays, like exon arrays or tiling arrays, address transcript expression analysis by an increased number of probes and respective selection of probe sequences.
Upon using selection pathways A, B, or C for tumor targeting, the enriched peptide sequences varied depending on the respective selection pathway, indicating that the most suitable screening conditions may have to be evaluated for each individual target tissue.
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Ask representatives from each group to explain the reasons for their respective selections.
Samples were then resuspended in DEPC treated water and desalted using G25 Sephadex spin columns (GE Healthcare, Buckinghamshire, UK) and then dialyzed against their respective selections buffers overnight at 4 °C.
We identified a panel of tumor-specific antibodies from the described libraries; these antibodies were reactive with ED-B domain, MUC1, CEA and MCF7 breast carcinoma cells used in the respective selections.
With regard to allele frequencies we asked the following question: are the genes for cooperation (C) and quorum sensing (S and R) selected for beyond their respective mutation-selection equilibria based on the metabolic selection coefficients sC = (MC −M0)/MC, sS = (MS −M0)/MS and sR = (MR −M0)/MR and the (uniform) mutation rate μ ?
Transformed bacteria were selected on LB-plates containing either ampicillin, or kanamycin depending on respective bacterial selection markers (Table 1).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com