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The back end of Saturday's first and second halves will tell us everything about how affected the two teams have been by their respective preparations.
To prepare the different mAb mass doses, non-radioactive J591 was added as necessary to achieve the final desired mAb mass after drawing up the 89Zr-J591 [15.7 9.0μCi (5.7–9.0 MBq) per animal] or 124I-J591 [171-26.3 9.9(6.3–9.9 MBq) per animal], based on the initial SA of the respective preparations.
The respective preparations did not induce apoptosis or necrosis in lymphocytes or DCs.
Oligomers of both Aβ isoforms in their respective preparations varied in size from dimers to 50-mers.
The binding functionalities of both mammalian and yeast derived preparations are gauged demonstrating the functional equivalence of the respective preparations.
The concentrations used in the in vitro experiment are comparable to the concentrations which are reached locally after subcutaneous injection of one ampoule of the respective preparations.
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Anti-Kv12.2 (1∶100), KCNE1 (N-16) or KCNE3 (N-18) (both Santa Cruz Biotechnology, CA; 1∶100) or anti-Kir2.1 (Chemicon CA; 1∶50) as a negative control, covalently conjugated to 20 µl of protein A/G sepharose beads (ProFound kit, Pierce, IL) were added to each respective preparation, and left overnight at 4°C.
The overexpression of rUbi and rMERS-NP were also visible in their respective crude preparations as a thicker band.
The initial counts of the bacteria and the phage were also verified later by culturing aliquots of the respective stock preparations obtained just prior to inoculating into the medium.
The respective RNA preparations were used in two DNA microarray hybridization assays by applying label swapping.
Functional vector titers were determined by transducing HEK293T cells with the respective vector preparations in a 10-fold dilution series.
More suggestions(15)
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com