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A major unsolved question when considering the autophagic functions of Bcl-2 and Bcl-xL was their respective localisation: Beclin 1 and hVPS34 are reported to localise at the trans-golgi network, while enforced ER-targeted Bcl-2 and Bcl-xL were reported to regulate autophagy [19], [24].
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Additionally, the respective subcellular localisation of CrSGD-GFP and GFP-CrSGD were confirmed within C. roseus leaf epidermal cells, i.e. the in planta CrSGD-expressing cells (Additional file 3a-h).
The six candidate genes, their respective chromosomal localisation as well as the frequency and rank of genomic and expression alterations, respectively, are summarised in Table 2. Examples of typical staining patterns from the antibodies used are shown in Figure 1.
Since co-immunoprecipitation in C2C12 cells confirmed a p55γ but not p85α interaction with BMPRII, we compared their respective co-localisation patterns in intact cells.
The findings are considered in relation to the essential components of the cognitive architecture of rhythmic function, and their respective cerebral lateralisation and localisation.
Analysis of tissue-specificity and localisation of the respective gene products to defined cellular compartments remain to be done.
The ProtComp server [ 19] was used to predict the sub-cellular localisation of the respective predicted proteins (of the isolated genes) (Table 1).
To map the endosome binding domain, we analysed the subcellular localisation of the respective mutated versions Upa1mP-Gfp, Upa1ΔN1-Gfp, -ΔN2 and -ΔN6.
In parallel, we determined the specific subcellular localisation of the respective human proteins, as the cellular environment contributes information on the possible suite of interaction partners a protein may have and consequently on its potential function(s).
Several pain localisations are possible.
This strongly suggests that the driving force for localisation of p53/p63/p73 to their respective sites in the genome is their sequence-specific binding.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com