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As osteocytes resorb the surrounding cortical bone, the shape of their respective lacunae might be expected to become irregular or elongated [ 35].
However, prior to undergoing apoptosis, osteocytes may be actively resorbing their respective lacunae, thus contributing to the baseline osteopenic phenotype of Cx43 deficiency.
Prior to undergoing apoptosis, osteocytes in cKO mice may be actively resorbing their respective lacunae via the process of osteocytic osteolysis.
In addition to osteoclast-mediated bone resorption, recent studies have suggested an important role for osteocytes in bone turnover and skeletal homeostasis via the process of osteocytic osteolysis – direct resorption of bone by osteocytes within their respective lacunae [ 25- 27].
Thus we were next interested to know if it was possible to distinguish the types of aggregates produced by measuring the sizes of the respective lacunae, approximated by simple histomorphometric cell surface area measurement on aggregate sections.
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