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cDNA sequenced peptides were attributed to their respective fragmentation spectra to confirm the structure of the final processed peptides.
While the respective fragmentation pathways were identical for all PCB TCE sulfate diesters investigated, the intensities of one fragment ion, [ArOSO3H]+, was significantly lower when two ortho chlorine atoms were present.
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The concentrations of O2, CO and CO2 were determined from the respective AMUs using standard fragmentation patterns and sensitivity factors determined by analyzing calibration gas mixtures.
We also tested other hypotheses that failed to disclose further differences or potential explanations: For instance, searching the data with modifications corresponding to in-source fragmentation of the respective label in various forms failed to identify additional hits.
The T → A transversion of the nonsense mutation introduces an AvrII restriction site and AvrII digestion of the PCR fragment results in fragmentation patterns which are indicative for the respective genotype of the tested animals.
The segregation and fragmentation of the respective khanates in the Mongol Empire were not the only factors in contributing to the decline of the Pax Mongolica.
The outcome of this putative identification was further validated by the fragmentation pattern of the respective standards of the same set of metabolites (Additional file 3).
A series of diblock copolymers bearing strong push pull azo chromophores and cholesteryl groups on the respective blocks was synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization.
The identification of active compounds was on the basis of their fragmentation pattern, literature data, and respective standards comparison for both MS and TLC.
Oxygen and nitrogen nucleophiles were insufficiently nucleophilic at physiological pH to react with NBD-Coum, which indicates that fragmentation of NBD-Coum into its respective NBD and coumarin components requires SNAr by stronger sulfhydryl-containing nucleophiles.
Employing this information, the algorithm is capable of unambiguously annotating fragment signals of the respective precursor ions without the need of spectral comparisons to tandem mass spectra libraries or the need of in silico fragmentations of substances under investigation.
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