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Other genes must be involved in the respective fate determinations at their specific progenitor areas.
During embryogenesis, neighboring progenitor cells in a developing field are able to discern incremental changes in the Shh signal strength and adopt their respective fate accordingly (Ribes and Briscoe, 2009; Balaskas et al., 2012).
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The power of bifocal 4D-microscopy becomes evident when individual cells, their respective fates, and movement patterns at different developmental stages are compared (Fig. 5 and Additional file).
Interestingly, it is at this stage that PrE and epiblast cells have been shown to be completely committed to their respective fates (Gardner and Rossant, 1979; Grabarek et al., 2012), indicating overt polarisation of aPKC is a hallmark of epithelialisation in the PrE and correlates with cell fate stabilisation.
Because no mesenchymal cells, such as blood cells or tunic cells, are present in the adult larvacean and because the heart, surrounded by purely epithelial cells, is the only mesodermal structure in the adult larvacean [ 23, 24], we suspect that all descendants of B5.1/ B5.1 are purely endodermal in their respective fates.
Figure 3(b) shows the sublineage of respective VPC fate according to the criteria defined by Sternberg and Horvitz [ 32].
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