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In these two cases they tend toward but do not reach the respective empty or half-filled level.
The resultant plasmid (pTCK303-dsRNAiTCD10 and CRISPR/Cas9 expression) and the respective empty vectors were introduced into Agrobacterium tumefaciens EHA105 and used to infect calli of WT plants according to the previously published methods (Hiei et al. 1994).
In both cancer cell types, introduction of NES-hTERT significantly decreased the number of colonies formed, suggesting decreased tumorigenic potential of those cells compared to the respective empty vector-expressing controls.
The strain was sequentially co-transformed with pRHF-luc (carrying the PFUS1-luciferase reporter module), pRGP-Gpa1 (for Gpa1 expression under its native promoter from a low copy plasmid; [19]) or the respective empty parent plasmid pRS413 [48], and the pYES2-based receptor expression constructs as indicated in the results section.
All cDNA transfections were carried out in parallel with the respective empty vector as a control.
Promoter regions with at least 1.5-fold enrichment over the respective empty vector control were retained for further analysis.
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As shown in Figure 6a, SR-I κB α-expressing PC12 cells show a significant and sustained increase in phosphorylated JNK1/2 levels (P-JNK1/2) after 24 h of TNF α treatment, as compared with their respective empty-vector control.
Brain glucose metabolism in both groups was compared to their respective control conditions (empty bladder).
Both CCK and gastrin levels increased after the meal, with no differences between subjects with normal respective delayed gastric emptying.
The proteins were tested for autoactivation by cotransforming their respective plasmids with an empty prey or bait plasmid.
To generate lentiviral particles, HEK 293T cells were seeded in 25 cm flasks at 1.3 × 10 cells per 4 ml and transfected the following day by the calcium phosphate method with 4 mg of pLKO.1-puro carrying the respective shRNAs or with empty pLKO.1-puro.
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