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Respective concentrated serum-free media served as reagent control and control OD values were subtracted from those of all other samples.
Cells were harvested after 24 hr by centrifugation, washed twice in the ninefold concentrated supernatant of either the gadusol-producing strain BY4742 tal1∆ trp1∆/pXP416-MTOx trp1∆/pXP416-MTOxf the control strain BY4742 tal1∆ trp1∆/pXP416, pXP420, and suspended in the respective corcentrated supernatants at 10 cells/ml.
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I concentrated and concentrated.
We assumed that 80% of enrolled participants would truly have tuberculosis [ 20- 27], that 50% would be HIV-infected [ 28, 29], and that the sensitivity of un-concentrated AFB smear for pulmonary TB was 38% in HIV-uninfected persons and 29% in HIV-infected persons (respective sensitivities of 50% and 38% were assumed for concentrated smears).
The results of the theoretical analysis for discs made either from Dionysos marble or PMMA are considered in juxtaposition to the respective ones obtained for uniform radial pressure as well as for concentrated point load.
The ST34 and ST5 strains cultured in the respective carbon sources in the 96 deep-well plate were acidified with concentrated hydrochloric acid (HCl, Merck, Darmstadt, Germany) to a pH of approximately 4 and were allowed to stand at ambient temperature for 24 h.
The 100 mg and 50 mg concentrated methanol and n-hexane extracts of phytococktail were dissolved in 25 ml of respective solvents, vortexed properly and filtered through 0.22 μm syringe filter (Millipore Corp., Bedford, MA, USA).
Each fish sample (whole fish) was then blended and then put back into their respective vials and then digested using the nitric acid digestion method (APHA 1992) by adding 5 ml of concentrated HNO3 and 1 mL of H2O2 analytical grade quality and heated on a microwave for several hours at about 70 °C.
Medicaid and Medicare, the government's, respective, mean-tested and entitlement, health care programs were specifically designed to mediate the effects of such concentrated impoverishment.
Approximately 30 µl of concentrated sample was electrophoresed on 10% SDS PAGE gels as previously described (23), and immunoblotted using the respective antibodies as indicated.
More concentrated.
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