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Dota 2 features the factions of the Radiant and the Dire in the stead of the Sentinel and the Scourge respectively, with the characters' respective alignment preserved, while also re-establishing their individual character traits in a stand-alone form.
The nuclear genes contained many indels within coding regions, so following the removal of introns, the exon regions were aligned against the respective alignment of amino acid sequences using ClustalW as implemented in DAMBE 4.2.13 [ 71].
However, a glutamate occurs with same frequency at the respective alignment position in the SP family.
We have used six sequences in our analysis and ClustalW (ver. 1.83) was used to elucidate respective alignment scores.
Using the multiple sequence alignment technique, we have observed the similarity in the sequences and their respective alignment scores were elucidated.
Sequence similarity plots are displayed in the LC Bs and the height of the sequence identity plot reflects the average column entropy for the region of the respective alignment.
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For a sample of 50 genes given in Table 3 the presence of recombination in the respective alignments was also manually confirmed using RDP3 [63].
The Analyst also reported the coverage of all coding genes in D. simulans and D. sechellia and calculated the divergence between the pairs mel-sim, mel-sec and sim-sec by using their respective alignments.
Missing sequences for certain taxa in certain partitions were coded as missing data in the respective alignments.
In multiple analysis, trees are inferred separately from the respective alignments and only relationships that are well supported in all (or most) of the trees are accepted.
Here, trees are inferred separately from the respective alignments and only relationships appearing in all (or most) of the trees are accepted (see also [ 34]).
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