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Since the optimal alignment is not guaranteed to be the correct one, it is expected that even the best alignment will contain sites that do not respect the assumption of positional homology.
Their mapping in metagenomics seems easier due to their relative smaller size, but they are constrained to respect the assumption of co-linearity, and therefore have to rely on the performance of metagenomic assemblers, that though promising often implement conservative assumption and potentially fail calling all the genes of an operon as present.
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In this context, it is worth emphasizing that interpretation of our findings should respect the assumptions and limitations of the models used in estimation.
For these datasets the acquisition path has been rearranged (respecting the assumption of partial overlap with at least one of the previous range images in the path).
When proffering solutions that touch entitlements, respect the reflex assumption that any change could erode individual benefits.
The patients' responses to the items were obtained with a LPCM in order to respect the three assumptions of the Rasch-family models [ 32].
Logarithmic transformation of the measured dose was required in order to respect the model's assumption of normal distribution of residuals.
To respect the proportional hazards assumption, analyses were performed according to 5-year interval birth cohorts using the STRATA option of SAS PHREG procedure (SAS 9.1.3; SAS Institute, Cary, NC).
Only two variables, septicaemia and acute upper respiratory infection, didn't respect the time-stationary assumption and they have been removed from the model under study because of their low frequency in our sample, although they could be very important risk factors for hospital readmission.
At all times data respected the normality assumption.
This paper is based on the following assumptions, which respect the real nature of the system: (1) The rack is considered to be a continuous rectangular pick face where drop-off station is located at the lower left-hand corner.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com