The unpublished linkage map contained 858 microsatellite markers, including 271 BAC-derived microsatellite markers, which make it good resource for the physical map assessment.
The high-density and the relatively high-resolution genetic map provides valuable resource for integrating the physical map and whole-genome assemblies.
Further integration of other existing genomics resources, such as ESTs, into the physical map should enable better understanding of the molecular mechanisms underlying adaptive radiation and elaboration of floral morphology.
In order to fully utilize the physical map as a resource in genetic investigations, comparative analyses, and whole genome sequence assembly, we end sequenced the 13,614 fingerprinted BAC clones that were used to generate the physical map.
The large number of new SSR markers and their placement on the physical map represent a valuable resource for studying diversity, constructing genetic maps, functional gene mapping, QTL exploration and molecular breeding in foxtail millet and its closely related species.
Alignment of this physical map with a genetic map of Brachypodium [15] identified long-range collinearity between the physical and genetic maps, thereby supporting the physical map assemblies and providing a useful resource for map-based gene isolation by aligning BACs with genetic markers and recombination data.
For G. soja, the physical map provides an anchored, clone-based resource to shuttle between the two genomes, domesticated and undomesticated.
These sequence resources represent tools for in silico biology that can resolve the physical map and de-convolute the complete genome sequence.
We also demonstrate essentially complete coverage of chromosome arms by the physical map and sequence assemblies, and provide an important resource for gene isolation.
Thus, the physical map described here integrates across a number of studies and genomic resources for Aquilegia.
