As the protein concentration increased and steps became difficult to resolve, step speeds could no longer be measured.
Our general approach is to kinetically resolve steps in the pathway of viral envelope glycoprotein-mediated membrane fusion and to uncover physical parameters underlying those steps using a variety of biochemical, biophysical, virological, and molecular and cell biological techniques.
AJ: Yes, with the India and Pakistani relationship, we cannot resolve the Kashmir issue through war, and we must begin to recognize that, and it can only be resolved step-by-step, and step-by-step resolution comes if you are talking to each other.
Several studies have already resolved steps in the early stage of biosynthesis of the vitamin [18], [22], [31], [35], [36].
In 6 experiments, we observed lamellipodium increasing the exerted force in well resolved steps of approximately 0.2 pN, corresponding to displacements of approximately 18 nm (Fig. 4d).
We employed single-molecule fluorescence resonance energy transfer methods to resolve steps during DNA unwinding by T7 helicase.
By varying dosage through a series of clearly resolved steps, we captured dynamics obscured in previous gene-deletion studies.
Also, some sulfur compounds can be oxidized abiotically adding further difficulties in resolving enzymatic steps from chemical changes.
Our study highlights the explanatory power of resolving the steps of the infection process in order to better understand host-parasite interactions and coevolution.
It is only once you know a problem exists that you can resolve it, step-by-step," shares Mayor VladanVasic.
