Sentence examples for resolving buffer from inspiring English sources

The supernatant of each sample was loaded onto a continuous 15% - 45% (w/v) sucrose gradient in high salt resolving buffer (20 mM HEPES (pH7.4), 150 mM KCl, and 10 mM MgCl2) generated by a Biocomp gradient master.

Pellets were dried at 25°C and resuspended in 20 µL loading buffer before resolving them by SDS-PAGE.

Washed beads were eluted with LDS sample buffer before resolving by SDS-PAGE, transferred to nitrocellulose membranes, and probed for FLAG-tagged receptor (M1 antibody; Sigma).

Pellets were washed carefully with F-buffer and resolved in SDS sample buffer.

After extensive washing in lysis buffer, precipitates were resuspended in SDS sample buffer, resolved by SDS-PAGE, and analyzed by Western blotting employing standard methods.

Immunoprecipitated protein complexes were washed four times in lysis buffer, eluted by heating in LB sample buffer, resolved on SDS-PAGE and immunoblotted with the indicated antibodies.

After rinsing five times with the lysis buffer, complexes were eluted in non-reducing sample buffer, resolved by SDS-PAGE, and immunoblotted with mAb 5A6.

The bead fractions were resuspended in SDS sample buffer, resolved on 4 20% Tris-glycine SDS-polyacrylamide gels, and analyzed by both phosphorimaging, as well as transfer and anti-FLAG immunoblotting (see below).

Short 6/8 fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition.

The protein flow through was resolved in RIPA buffer with 10% SDS.

Immunoprecipitates were then washed in lysis buffer, resolved by SDS-PAGE, and analyzed by immunoblotting.