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He again became interested in virtuoso technique and resolved to transfer some of Paganini's fantastic violin effects to the piano, writing a fantasia on his La campanella.
In 1994 the Kazakh government resolved to transfer the national capital from Almaty to Aqmola, a process completed in 1997, and the city's name was again changed the following year.
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Each protein sample (30 μg) was resolved to SDS PAGE, transferred onto a polyvinylidene difluoride membrane (Millipore, Molsheim, France) and incubated with a monoclonal antibody against βIII-tubulin (1 : 10 000) or β-actin (1 : 16 000; AC-15; Sigma-Aldrich).
They resolved to decriminalize all drugs, and transfer all the money they used to spend on arresting and jailing drug addicts, and spend it instead on reconnecting them -- to their own feelings, and to the wider society.
Samples (30 μg protein) were resolved to 10-15% SDS-PAGE and transferred to a nitrocellulose membrane.
The legal obstacle to federal aid was resolved by gaining approval to transfer the funds.
One way to resolve this ambiguity would be to transfer each secondary mutation to a series of re-isolated dps-deleted clones.
Total cell lysates were then subjected to SDS-PAGE and the resolved proteins transferred to a PVDF membrane.
Proteins were resolved and transferred to Immobilon-P membrane (Millipore, Billerica, MA) using semi-dry transfer (Bio-Rad, Hercules, CA).
Protein concentration was determined by Bradford assay and equivalent quantities of protein were resolved and transferred to PVDF membranes, which were then blocked (2 hrs at room temperature) in PBS containing either 5% nonfat milk, 10% nonfat milk, or 5% BSA depending on the antibody.
The heated samples were loaded onto 15% SDS gels, resolved, and transferred to nitrocellulose membranes using 60 mA for 90 minutes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com