The ambient mass spectrometry technique, desorption electrospray ionization mass spectrometry (DESI-MS), is applied for the rapid identification and spatially resolved relative quantification of chlorophyll degradation products in complex senescent plant tissue matrixes.
This provides a significant increase in the number of cells that can be acquired, but sacrifices the ability to resolve individual fusion events, while maintaining the highly resolved sub-cellular quantification (Lovy et al. 2012).
In principle, the model we propose can be generalized to other systems through dense taxon sampling, a resolved phylogeny, and quantification of alternative morphologies.
Cytokines secreted during osteoclastogenesis were resolved using multiplexed quantification combined with a Partial Least Squares Regression model to identify the relative importance of specific cytokines for the osteoclastogenesis outcome.
a Binary images showing regions of interest in regular looking vasculature, b irregular looking vasculature over the course of RF field exposure, and c time-resolved quantification of % area fraction during RF exposure in regular and irregular looking vascular.
a Binary images showing regions of interest in regular looking vasculature, b irregular looking vasculature over the course of RF field exposure, and c time-resolved quantification of % area fraction during RF exposure in regular and irregular tumor vascular.
Both, depth-resolved quantification and removal of the effect of thickness variation are achieved by exploiting the coherence gating implicit in the spectral-domain interferometry.
High-content single cell microscopy enabled the time-resolved quantification of both the population of monocytic precursors and the emerging osteoclasts.
