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Several HJ substrates were analyzed for cleavage and incision mapping, showing that BTBD12 and associating proteins have divergent incision patterns, and only 25% of resolved products are religatable (Fekairi et al. 2009; Munoz et al. 2009; Svendsen et al. 2009).
Multiple faintly stained and poorly resolved products ranging between ∼1.0 kb and ∼2.0 kb were amplified from Ms17 roots, and multiple products ranging from ∼1.0 kb to ∼1.8 kb in Ms17 shoot tissue.
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Assimilation of the sugars by the fungus resolved product inhibition to enzyme caused by accumulation of them, and consequently, the sugar production in SSF was smoother than that in hydrolysis reaction without the fermenting strain.
An ABI 377 prism automated sequencer was used to resolve products, which were then analysed using GeneScan v3.1.2 and Genotyper 2.5 (Applied Biosystems).
Initially, the most frequently used seven methods of complexity reduction (all based on a combination of PstI RE and a single 'frequently cutting' RE) were tested in several Brassica accessions by resolving products of representation amplification on 1.2% agarose gel.
The settlement, according to Toyota's statement, resolves product liability claims by the Saylor and Lastrella families against Toyota and the dealership.
The resolved PCR products were extracted from the gel, purified using QIAquick PCR Purification kit (Qiagen, Germantown, MD) and sent for sequencing.
Primers were verified by performing PCR with control DNA (isolated from TK6 cells) and resolving the products on agarose gels.
Agarose (1.2 %) was used for resolving PCR products.
A 3% agarose gel was used to resolve PCR products (130 V; 20 min).
Amplification was confirmed by resolving PCR products on a 1.5% agarose gel.
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