Equivalent results were obtained when 660/20-A was combined with SSC-A (Figure 4E, right panel, P3, P4 and P5, P6), i.e, rather than showing a two-step separation resulting in four clearly resolved populations, the addition of the 660/20-A autofluorescence parameter did not significantly enhance the separation achieved by the scatter gating parameter alone (see also Figures S3 and S4).
On a plot of Glast vs CD24, live SVZ cells should have two clearly resolved populations that are either CD24mid or CD24−/low.
Population genetic analyses were conducted using the putative mitochondrial control region [56], a 941-bp fragment that has been shown to have high haplotype diversity and the ability to resolve population genetic structure in A. cervicornis across the Caribbean [21].
It is possible that this pattern reflects the greater sampling effort at the North Wales site and the concomitant statistical power to resolve population structure (i.e. ability to model linkage disequilibrium and Hardy-Weinberg equilibrium conditions) at this location as compared with elsewhere (see [36] for methodological details).
In these circumstances, data from thousands of markers (genomic data) may be necessary to resolve population structure and aid management.
Second, coalescence analysis of the observed and simulated sequences was unable to resolve population histories prior to the last glacial cycle.
BAPS had some difficulty resolving population structure and provided nearly equivalent support for either K = 4 (P = 0.503) or K = 5 (P = 0.497).
This study aims to resolve population genetic patterns of walleye across its native range, providing a baseline for evaluating future anthropogenic pressures.
Autosomal markers can resolve population structures in bank voles at larger geographical scales (White et al. 2013), but populations may undergo very specific processes at the local level, for example, source sink dynamics (Guivier et al. 2011) perturbing spatial patterns.
Unfortunately, the power of microsatellite data to resolve population structure in this species may have been compromised for the comparison between East Australia and Indonesia, as sample sizes were low, but sample sizes were adequate for the comparison between West Australia and Indonesia.
