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Asn179 and Asn220 are adjacent to the disordered region and are not resolved in this structure.
The N-terminal MBD, adjacent to the MA helix, was not resolved in this structure.
Eight Mg2+ ions were resolved in this structure, two of which appear to mediate interactions between RNA molecules in the crystal lattice, giving six Mg2+ ions playing structural roles in the ribozyme.
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Domain V and part of domain IV were not resolved in this crystal structure and are not shown in the schematic.
Remarkably, this loop of about 11 amino acids (DGSKHVEPVRN) is not fully resolved in the structure of the AtLOV2 domain [ 14].
Large side chains of protein and RNA bases are resolved in the structure core, whereas some peripheral regions including many AFs are of lower resolution (Figs. S3 and S4).
These helices are better resolved in the structure of p110γ bound to ATP.
Other residues including S339/S340 in human NHERF1 are either disordered or not resolved in the structure [ 29].
The C-terminal amino acid leucine is extended by three amino acids not resolved in the structure, and, in LDHBx, by an additional seven amino acids.
The last residue at the C-terminus of the first SUMO monomer in the proposed di-SUMO2 and the first N-terminal residue of the second SUMO monomer that are resolved in the structure are 14.1 Å (1 Å=0.1 nm) apart.
Available crystallographic data for TYK2 indicates a cis arrangement (although the interdomain linker is not resolved in the structure) [ 71], as does recent molecular dynamics modelling of JAK2 supported by limited charge reversal mutagenesis [ 72].
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