In Crambidae, relationships of the five (of 14) subfamilies sampled were also strongly resolved, corresponding well to the morphological hypothesis of Yoshiyasu [ 67], less well to that of Solis and Maes [ 16].
Seven major bands were resolved, apparently corresponding to PSII supercomplexes, PSI-light harvesting complex (LHC) I, PSI core, ATP synthase-Cytb6f-PSII core, CP43 less PSII core, LHCII trimer, and dimer.
Three linkage groups were resolved each corresponding to one of the three chromosomes (not shown).
Alleles at individual loci were widely distributed among the named species but this distorting effect of recombination was largely buffered by using concatenated sequences, which resolved clusters corresponding to the three species most numerous in the sample, N. meningitidis, N. lactamica and N. gonorrhoeae.
This resulted in the identification of 16 Lox2 resolved clones and 3 Lox2ΔG resolved clones, respectively corresponding to 0.74 and 0.26% of the screened colonies.
Where the strains analysed were more numerous, particularly in lineage IV, the phylogeny was resolved into clusters corresponding to strains with the same regional localisation.
Combined with RNAseq data, this data led to a significant improvement of the reference genome sequence by resolving 1194 gaps corresponding to 460 kb and resulting in a remarkable improvement of the 7699 protein coding genes models.
The HECT-Smurf2 mutant contains no WW domains, and GST pulldown followed by anti-HA Western blot did not resolve a band corresponding to HECT-Smurf2, indicating that in the absence of WW domains, Smurf2 does not interact with RNF11.
The Zn 3p binding energy of the ZnO nanorods can be resolved into two peaks corresponding to Zn 3p 3/2 (90.1 eV), Zn 3p 1/2 (93.1 eV).
