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We sequenced and assembled a new mt-genome for Paraplea frontalis (Fieber, 1844), except for small portions of 12S rRNA gene and the control region (polynucleotide sequences in these two regions proved difficult to resolve with certainty).
The promise of phylogenomics is that the ability to collect many orthologous loci for taxa of interest will increase the resolution of the Tree of Life, even for nodes that have historically been difficult to resolve with certainty.
Information in these databases is often hard to resolve with certainty because of subsetting different data in a variety of formats; use of different bioactivity metrics; use of different identifiers for chemicals and proteins; and having to access different query interfaces, respectively.
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Most of the nodes received strong bootstrap support, but we also identified nodes that require more scrutiny and probably denser taxon sampling to be resolved with certainty (for a detailed genome-based overview of agaricomycete phylogeny, see Supplementary Note 1).
That question may never be resolved with certainty.
This extreme rate heterogeneity precluded resolving with certainty the exact phylogenetic placement of Aplousobranchia.
Because the evolutionary origin and diversification of proeukaryotes is not yet resolved with certainty [ 35- 37], this limits our ability to discuss the evolution of HTPG.
Phylogeny and character evolution in the Bennettitales, Erdtmanithecales and Gnetales are currently only partly understood and under debate; the exact systematic position of Siphonospermum simplex, i.e., its position within the Gnetales, cannot be resolved with certainty.
Siphonospermum simplex clearly constitutes a new evolutionary lineage within the Gnetales, perhaps sister to Gnetum or to the Gnetum-Welwitschia clade, but its precise systematic position can currently not be resolved with certainty.
Unfortunately, the sequence gap between CBX6 and CBX7 is not resolved with certainty in the opossum and in the platypus genomes, and we cannot confirm whether the last common ancestor of Eutheria and Metatheria already possessed a single ancestral A3, the intermediate stage with two loci, or already three loci, as the ancestor of all placental mammals.
For example, suppose that pre-approval clinical trials had raised suspicion that a new drug might pose a particular kind of risk, but not enough information could be gleaned from the trials to resolve the question with certainty.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com