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The paired-end reads were generated to resolve the assembly problem caused by repetitive regions.
A specific strategy to resolve the assembly of phage-related regions was developed which generated candidate molecules for each strain, thus making possible the identification of prophage-like elements and phage remnants (data not shown) In the same way, identification of all predicted integrase genes and prophage-like regions were carried in Xf-CVC 9a5c and Xf-PD Temecula strain genomes.
PCR was also used to resolve the assembly of the D1 region.
We examined whether increasing stringency for FPC construction of the physical map could resolve the assembly in problematic contigs.
The introduction of short sequence tags to fragmented BAC DNA prior to pooling and sequencing helped to resolve the assembly of multiplex sequencing data by establishing relationships between BAC clones and sequence reads, reducing sample complexity.
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An assembly representation model, a feature-based hierarchical co-assembly representation, is proposed and a new definition of the assembly feature is given to resolve the co-assembly design issues.
It appears unlikely that algorithmic advances will allow us to resolve the self-assembly of layered nanocomposites on an all-atom level in the near future, primarily due to limitations in clock speed of single computing cores.
To improve the assembly, resolve the Nst1 and Acd1 genomes and correct artifacts we combined several approaches.
Work is ongoing to resolve the gaps in the genome assembly of chromosome 5.
Novex 8 16% minigels (Life Technologies) were used to monitor protein purifications and to resolve the products of all EspP assembly reactions.
In other cases, ambiguities cannot be resolved and the assembly cannot be completed.
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