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While a very powerful technique, SPA still suffers from two primary limitations: first, it is sometimes unable to unambiguously resolve reliable structures of macromolecules exhibiting continuous conformational flexibility; second, it cannot be directly used to study macromolecules within cells or other unique structures in situ.
However, short 16S rRNA gene amplicons are frequently inadequate to resolve reliable species-level identifications (Bokulich and Mills, 2012a).
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Such a problem can only be resolved by reliable politicians.
"Such a problem can only be resolved by reliable politicians," Fleischer concludes.
Although cMap has been widely applied, problems remain to be resolved for reliable prediction.
Our simulations therefore suggest that S uperT riplets infers less-resolved but more reliable conservative supertrees, whereas b- and t-MRP infers more resolved but less reliable liberal ones.
Challenges still need to be resolved to build reliable, secure, and efficient e-health platforms with great flexibility.
Elasto-plastic, viscoplastic, and creep problems, however, are nonlinear, and this gives rise to a number of questions that must be resolved before a reliable solution can be established.
4. Using these assumptions we will explore several models to determine linear range and minimal reliable resolved concentration fold change.
In both cases, S uperT riplets tends to propose less resolved but more reliable supertrees than those inferred using M atrix R epresentation with P arsimony.
A more definitive demonstration of a qualitative distinction between the properties of the slow exchanging (τ≈20 s at 25°C) major and minor species comes from the R2 relaxation rates for the minor species resonances that are sufficiently resolved to enable reliable peak integration.
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