Using a simple dry fractionation process under controlled conditions, palm oil can be resolved into two fractions, namely olein (liquid fraction) and stearin (solid fraction) [ 16].
The urokinase peak pool from Cu II -IDA-polyacrylamide Cu II -IDA-polyacrylamide Cu II -IDA-polyacrylamideate fracryogelfor high and low molecolumnweight forms of urokinase by gel filtration couldatography on Sephacryl S-200.
Extracts 1 to 6 were resolved into two fractions, referred to as anionic and cationic, by anion-exchange chromatography.
Extracts 1 to 6 were further resolved into two fractions, named anionic and cationic, by anion-exchange chromatography (Additional file 1: Figure S2).
Samples were acidified by addition of 2% (w/v) acetonitrile, 1% (v/v) trifluoroacetic acid, 0.5% (v/v) acetic acid, then the three differentially-labeled peptide pools were combined and resolved into 5 fractions using C18-SCX-C18 C18-SCX-C18 C18-SCX-C18
The sample was then resolved into 96 fractions by a HPLC system equipped with a C18 column, an Agilent1100 instrument, a quaternary pump, a manual injector, a UV detector and a workstation for peak identification and integration (Agilent Technologies, USA).
Many of these fractions resolve into inconvenient decimal series, necessitating shorthand or estimations.
Soluble chromatin fragments, obtained by mild micrococcal nuclease digestion of purified nuclei, are resolved by density gradient centrifugation into fractions of homogeneous composition and precise molecular entity, from mononucleosomes to oligonucleosomes [20].
Using SPE method described in Ong [ 10], fraction A was further resolved into four purer fractions AA, AB, AC and AD.
Typical biofluids and tissues are very complex mixtures which can be resolved into defined molecular fractions using current high-performance separation technologies.
The CB peptides were initially resolved into two major fractions by chromatography on a Bio-Gel P-2 column (100 200 mesh, exclusion limit 2600 Da, globular proteins; Bio-Rad), equilibrated with 0.1 M acetic acid.
