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Additional nucleic acid testing (NAT) is recommended to resolve indeterminate or undifferentiated HIV seroreactivity.
To confirm or reject the results of the screening assays and to resolve indeterminate results, supplementary tests, such as western blotting (WB) and peptide ELISA are used [ 6].
The strengths of this study are its use of a standard WHO design for evaluation of RDTs, as well as the use of DNA PCR to resolve indeterminate OIC and Western Blot results.
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Clinical laboratories should keep this in mind and consider retesting patients accordingly if alternative methods are not available for resolving indeterminate samples.
Data were analyzed retrospectively to assess consistency of test results between routine PMTCT and ANC surveillance data, with the surveillance algorithm using second generation antibody tests and no tie-breaker for resolving indeterminate results.
Additional deep or whole genome sequencing may resolve such indeterminate classifications, but was beyond the scope of this study.
It is possible that we would have been able to resolve the 4 indeterminate samples had we retested these individuals 2 to 4 weeks later, following the manufacturer's guidelines; this resolution might possibly have improved the concordance for the Novagnost assay.
The gold standard test was the Western Blot, with indeterminate results resolved by PCR testing.
Indeterminate results were resolved using Biotest EIA (Biotest Diagnostics Corp., Denville, NJ).
Indeterminate results were resolved using ELISA "Organon Uniform II" (Organon Teknia Ltd ,Boxtel, the Netherlands).
Indeterminate results were resolved by testing of a further serum sample obtained at least two weeks later.
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