In the first stage, they resolve the assignment problem using a scheduling algorithm which is the Longest Processing Time algorithm.
Overall, in addition to the Ile → Val mutants, we also utilized several 'nudge' mutations, which helped to resolve assignment ambiguities.
A common complementation of this procedure is using synteny-based methods which exploit synteny information to resolve ambiguous orthology assignment between genes in different species [ 34].
The connection domain S379C nudge mutation helps to resolve the assignments of Ile375 and Ile329 and also supports assignments of Ile341 and Ile382.
To resolve ambiguous assignments, juveniles exhibiting at least one mismatch when compared to each of the cross type families (N = 228), and those assigning at all loci to more than one parental cross, were genotyped at two additional loci (data not shown), as were all of the parents.
To help resolve ambiguous assignments, 2D N-TROSY-HSQC spectra were also recorded on 1 mM samples of [N-glycine,N-serine,N-tryptophan]HscB, [U-N,N-histidine]HscB, [U-N,N-lysine]HscB, [U-N,N-arginine]HscB, and [U-N,N-threonine]HscB.
The access-order information effectively resolves the assignment issue of the same secondary ligatures with the same position information to the same primary ligatures as summarized in Fig. 15.
The helical arrangement of actin subunits is now resolved, facilitating assignment of particular cross-bridge forms to specific actin subunits within the 38.7 nm repeat that spans from one Tn complex to the next.
Given the genome structure of Brassica, some scaffolds will be in repeat-rich or duplication regions, and thus it is difficult to resolve the LG assignments.
In the two discordant cases (7% of the evaluated gene pairs) intron evidence can be used to resolve the conflicting assignments made by Inparanoid and local synteny.
