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The resulting 7.6-Å resolution structure of BK and 9.1-Å resolution of the virus-like particles are the highest-resolution cryo-EM structures of any polyomavirus.
Super-resolution imaging of virus-infected cells revealed rod-shaped MAVS clusters on mitochondria.
In a second step, perfomed on cell monolayers in the absence of selection, another recombination event occurs, resulting in the resolution of the intermediate virus and leading to the formation of either a virus lacking the targeted gene or to wild-type virus.
Interestingly, KIR2DL3 and HLA-C1 have also been shown to play a role in the resolution of hepatitis C virus (HCV) in the context of low infectious doses of HCV but not in individuals with high-dose exposure [22].
On the morning of Friday, March 30, Rory had a bout of diarrhea which actually reassured his parents because this had been predicted by the ED physician as part of the likely resolution of his gastrointestinal virus.
For example, the presence of KIR2DL3 and its HLA-C1 ligand directly influenced resolution of hepatitis C virus infection [ 11] and herpes simplex type 1 infection was modified by the high-affinity receptor/ligand pair KIR2DL2/HLA-C1 [ 12].
This protocol requires that the viral capsid is icosahedral and has been reconstructed at a high resolution from cryo-EM images of virus particles, of which the orientations and centers used for the capsid reconstruction have been determined by virus reconstruction software packages, for example, RELION (Scheres 2012), EMAN (Ludtke et al. 1999), and FREALIGN (Grigorieff 2007).
We have used cryo-electron microscopy combined with three-dimensional reconstruction techniques and complementary biophysical techniques, to determine the structure at 26 Å resolution of the Penicillium chrysogenum virus (PcV) capsid.
