Sentence examples for resolution of strains from inspiring English sources

An analogous situation was found when IGS typing was used to characterize other microbes such as Fusobacterium spp.; in that situation, good resolution of strains was provided; however, with IGS typing, 2 species appeared to be identical (23 ).

The poor resolution of strain relationships in H. influenzae prevented identification of non-competent lineages that have persisted for longer periods of time, indicating that phylogenetic methods may not be well suited for tracing competence evolution in H. influenzae.

Overall, analysis of secondary metabolites provided finer resolution of strain differentiation than 16S.

The complete resolution of strain differences afforded by whole genome sequencing has shown how the genotype of M. ulcerans strains from two African countries correlate with place of origin.

However, 16S rRNA gene sequences showed limited variation in the closely related species of B. subtilis group (e.g. B. subtilis and B. amyloliquefaciens showed more than 99% similarities) and prevents the resolution of strain and species relationship [ 17].

The resolution of strain identification offered by MLST is such that different isolates sharing the same ST (genotypically indistinguishable at all 7 loci) are defined as clones of the same strain (e.g., for a group of isolates within this collection, we have further validated this identity by cloning and sequencing up to 10 random fragments of DNA).

The method is relatively straightforward and inexpensive when compared to other techniques, such as MLST, but clearly will not provide high resolution typing of strains if required.

At the resolution level of strains we see that even for an artificial metagenome where 16S rRNA genes are available, the S61 peptide approach can serve as a comparable tool for distinguishing strains from species.

However, Pathoscope did not provide accurate resolution of different strains in a sample, which is required for biosurveillance to distinguish pathogenic strains from non-pathogenic strains and to pinpoint the serotype of a pathogen from a complex metagenome background.

With this issue in mind, we already included targets other than the housekeeping genes, namely, the envelope proteins, LipL32 and LipL41 in our MLT scheme [8], [16]; this may allow sampling of variation beyond the core genome and which might be relevant in epidemiologic/taxonomic resolution of the strains.

As such, sequences of the HK genes, being representative of overall bacterial chromosomal change, can provide effective phylogenetic resolution of bacterial strains [ 26].