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We suggest that even if many traditional diversification hypotheses remain untestable, investigations using genomic datasets will provide greater resolution of species histories in the Neotropics and elsewhere.
The increased availability of genomic data, coupled with new computational methods, improves resolution of species relationships beyond what was possible in the past.
The kernel can be calculated numerically by Singular Value Decomposition (SVD) or determined in a symbolical way, the latter allowing the detection of particular stoichiometric conditions leading to spectral resolution of species.
For ITS locus, we examined the effect of trimming the ends on the resolution of species in the genus Agaricus.
To increase the descriptive resolution of species richness of the fish assemblages at each sampling location, belt transect surveys were supplemented by roving diver (RD) surveys to provide a more complete estimate of local species richness.
Inclusion of minor variants further improves the resolution of species identification [ 60].
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However, our results provide much greater resolution of species-specific associations of polyploid alleles within these clades.
While some species have little diversity among accessions and concur with the GBSSI and nitrate reductase results, such as S. agrimonifolium, S. colombianum, S. hjertingii, and S. moscopanum, the results give much better resolution of species-specific progenitors.
As expected, the combination of three markers (rbcL, matK, & ITS2) delivered both the highest overall species resolution (69%) and the highest resolution of congeneric species (41%).
This approach is also useful to understand the poor resolution of some species with broad distributions and recent diversification times (Clade I-B) vs. species with allopatric distributions and older diversification times (Clade II) that showed greater levels of mtDNA resolution and clustering.
Although 1 canonical SNP may be all that is required to identify a clonal species, subpopulation, and/or isolate (19 ), the massive number of potential SNPs in a genome provides incredible resolution of nonclonal species as well.
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