RPC design, gas mixture, limits on the bulk resistivity of ceramic electrodes, efficiency, time resolution, counting rate capabilities and ageing test results are presented.
Images were recorded using a K2 Summit direct electron detector (Gatan) in super resolution counting mode at a nominal magnification of 22,500× (rendering a pixel size of 1.32 Å) on an FEI Titan Krios electron microscope at 300 kV.
PALM, with the possibility of single-molecule resolution counting in sub-diffraction limit voxels, therefore clearly offers interesting prospects in this field.
Moreover, application of such devices in high-resolution counting systems increases the flexibility of the system, increases the degree of the system integration and leads directly to increasing the system reliability.
Examples of the use of potassium chloride as a tracer are given in the context of ion exchange and electrochemical migration studies, and comparisons in performance are drawn from literature with hyper pure germanium semiconductor detectors, which are more commonly utilised detectors in high-resolution counting applications.
A Gatan K2-Summit detector was used in super-resolution counting mode at a calibrated magnification of 35,714× (yielding a pixel size of 1.4 Å), and a dose rate of ~2.5 electrons/Å/s (~5 electrons/pixel/s).
We return to this issue now as recently Mancera et al. (2008) provided the first high-resolution counts of crossover and noncrossover recombination events in yeast (with a median of 78 bp between consecutive markers).
Spatial resolution, sensitivity, count rate and image quality were evaluated.
