This high-resolution consensus map included 9268 markers, consisting of 34 linkage groups.
Overlapping fragment patterns of separate DNA molecules were assembled into high-resolution consensus maps of the full-length chromosomes.
All 3D classifications and 3D refinements were started from a 40 Å low-pass filtered version of the high-resolution consensus structure.
The advantages of this immortal RIL population and its parental lines make it practical for high-resolution consensus mapping with additional sequence-based portable markers, enabling better understanding and exploitation of complex Gossypium genomes (Mace et al. 2009).
Those parts of the atomic model that were built in this structure but were disordered in the high-resolution consensus structure of the apo-state are shown in red.
When the high-resolution consensus map was low-pass filtered to 7 Å, besides density for TM2 a second, unidentified rod-shaped density was also observed in the cavity formed by TM2, TM3 and TM5 of PS1 (Bai et al., 2015a).
The mask for masked classification on the PS1 subunit (the red part of the signal in Figure 1) was generated by converting the atomic model of the PS1 subunit from the high-resolution consensus structure (including a poly-alanine model for TM2) into a density map using the program e2pdb2mrc.py from EMAN2 (Tang et al., 2007).
The arc of any genre is going to be contended, and the length of this particular arc won't be agreed upon without a fair amount of dissent (and neither resolution nor consensus is necessary).
The so-called stakeholders now have a place to come together for understanding of interests, of opportunities for compromise, and of conflict resolution through consensus agreement of certain modifications of behavior and patterns of use to reduce tension, disputes, and adamant, often political, resistance to change.
Subjective aspects have to be assessed independently by more than one reviewer with tracking of disagreements and resolution by consensus or arbitration.
Disagreements were resolved by asking the opinion of a third reviewer (IA) for resolution until consensus was reached at each stage of the screening process.
