However, choosing a distribution on the insert-length does not influence the expected resolution-ambiguity, only its variance.
For a fixed number of sequences N, increasing L increases the probability of detection, but also increases the resolution-ambiguity.
Define resolution-ambiguity as the size of the region θ (denoted by | θ|) in which ζ is constrained to lie.
For a fixed amount of sequencing, we confirmed the theoretically predicted boost in probability of detecting a breakpoint to within a resolution-ambiguity of 200 bp.
s < L' < L M : Once again, for inserts larger than the desired resolution-ambiguity s, their contribution to Pr(|Θ| ≤ s) is completely dependent on coverage.
For example, with an Illumina run with 2 Kbp inserts and 25 × 106 mappable reads one could detect nearly 100% of breakpoints with an average resolution-ambiguity of less than 500 bp.
We explicate the trade-offs between detection and resolution, and provide a method for computing the probability of SV detection as well as the expected resolution-ambiguity for a variety of technology and parameter choices.
Then, for an arbitrary s (see METHODS) (4) Note that the resolution-ambiguity |Θ| ≤ L1, or |Θ| = L2 can be obtained using single insert libraries, but the likelihood of resolving between L1 and L2 is optimized by using an appropriate mix of the two libraries.
Near-side pragmatics includes, but is not limited to resolution of ambiguity and vagueness, the reference of proper names, indexicals and demonstratives, and anaphors, and at least some issues involving presupposition.
Sequencing of Esmeraldo, a homozygous DTU IIb strain, was undertaken to aid in resolution of ambiguity in the assembly of the CL Brener genome.
Research examining the neurocognitive mechanisms involved in the resolution of ambiguity have sought to systematically manipulate the point in a spoken sentence where ambiguous material is resolved (Rodd et al. 2012).
