Exact(26)
A similar distinction was seen between differentiated cells and undifferentiated cells in mouse, human, and Xenopus model systems, so that in all cases cyclin E was easily extracted from progenitor cells but resistant to extraction in differentiated derivatives.
As a result, these dyes are the most resistant to extraction or destruction of all dye types.
Like the endogenous protein in human cells, ATAD3A-Myc-HIS expressed in yeast is found resistant to extraction with salt and certain detergents, suggesting membrane insertion.
Furthermore, many golgins are peripheral membrane-associated proteins that possess extensive coiled-coil domains and are resistant to extraction by Triton X-100 [5].
The remaining punctate cytoplasmic structures have been suggested to be aggregates resistant to extraction in similar experiments on other INM proteins [41].
Both the endogenous ZNF198 (data not shown) and the full-length Myc-ZNF198 (Figure 4A) were diffusely nuclear localized and this staining was resistant to extraction, consistent with ZNF198 being bound to chromatin.
Similar(34)
This polluted material, resulting from combustion in a light diesel engine and collected at high temperature inside the particulate filter placed just after the engine, was particularly resistant to conventional extraction techniques, such as soxhlet extraction, and had an extraction behaviour that differed markedly from certified reference materials (SRM 1650).
Although this scaffolding function was shown to be independent of CaMKIIα kinase activity toward Rpt6-S120, proteasomes containing the Rpt6-S120D mutant seemed to be more resistant to detergent extraction in hippocampal neurons (Djakovic et al., 2012).
These proteins are integral membrane proteins as they are resistant to alkaline extraction (not shown).
Results reveal that S184A, EA and AA mutants display more Bax proteins resistant to alkali extraction as compared to WT, S163A and S163E mutants (Fig. 4A).
As mentioned above [and see 4], neuropil glia also express neuroglian resistant to Triton extraction as they migrate to surround protoglomeruli.
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