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The dTALE gene dTALE-xa27, designed to target the susceptible allele of the resistance gene Xa27, elicited a resistant reaction in the otherwise susceptible rice cultivar IR24.
Genetic control of the resistant reaction in common bean can be controlled by different race-specific resistance genes depending on the bean genotype.
The expression levels of all the examined genes were induced or suppressed in either the resistant reaction or the susceptible reaction, or in both reactions.
The resistant reaction group was leaves from a 2-month-old Nipponbare-Xa21 treated with X. oryzae (resistant variety) and the susceptible reaction group was leaves from a 2-month-old Nipponbare-Xa21 treated with X. oryzae (susceptible variety).
Six days after inoculation, the host reaction was recorded using the Pan et al. (1996) scale, where a score of 0 2 was considered to be a resistant reaction and a score of 3 5 as susceptible.
The resistant reaction group was leaves from a 3-week-old Nipponbare-Pi9 treated with M. grisea (resistant variety) and the susceptible reaction group was leaves from a 3-week-old Nipponbare treated with M. grisea (susceptible variety).
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Most of the lines screened for seedling resistance against race TTKSK were susceptible, and only 15 (6 %) showed resistant reactions (IT 22+ or lower) (Fig. 1b).
Compared to Mangeumbyeo, the leaves of the NILs with Xa4, xa5 and Xa21 genes showed susceptible, moderately resistant and resistant reactions to the BB strains.
For these AGs, resistant reactions were never observed.
However, none evaluated presented resistant reactions (scores between 1 and 4), all having scored between 5 and 9.
Evaluation of all these advanced lines for blast resistance showed a differential reaction pattern to different isolates, with five out of seven entries showing resistant reactions and two showing moderately resistant reactions with different isolates.
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