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For resistance to spread the resistant, mutant parasites must survive both antimalarial drug effects and host-defence mechanisms.
A strain with a reverted phenotype (mainly concerning the resistance phenotype) was obtained from the resistant mutant.
Relative growth rates were calculated as r(resistant mutant) r(wild-type)−1.
The MPC for each resistant mutant and its respective wild-type were compared.
Fold change in IC90 was calculated as the ratio between the resistant mutant and its respective wild-type.
Some DANAs were also active at micromolar concentrations against the K103N + Y181C resistant mutant.
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In the present study, the Al-resistant mutant ral1 (resistance to aluminum 1) was selected for further characterization.
Through screening of ~5000 M2 lines, we identified 10 Al-sensitive mutants and one Al-resistant mutant ral1 (resistance to aluminum 1).
Asp84 in SNX2 was mutated to an alanine residue to generate the cleavage-resistant mutant SNX2.
It also sensitized cells to oxidant-induced death similar to the oxidation-resistant mutant.
Macvanin, M. & Hughes, D. Hyper-susceptibility of a fusidic acid-resistant mutant of Salmonella to different classes of antibiotics.
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