Sentence examples for resistant expression from inspiring English sources

pTZ-KanaGalK, was generated by sub-cloning the 2232 bp galactokinase prokaryotic expression cassette (GalK), along with the kanamycin resistant expression cassette (Kana), into the pTZ57R shuttle vector, cut with KpnI/PstI [ 14].

The first targeting vector, pTK-KanaGalK-TK, was generated by sub-cloning the galactokinase prokaryotic expression cassette (GalK), along with the kanamycin resistant expression cassette, into the pINT2 shuttle vector [ 25].

We, therefore, constructed a shRNA resistant expression clone of FBP1 (pCIA-CMV-FBPSHR) by point mutations in the shRNA targeted region in the central and C-terminal domains of FBP1 without altering the amino acid sequence.

Briefly, HeLa cells grown on cover slips were co-transfected with CD317 siRNA and CD317-HA-siRNA resistant expression constructs (X-tremeGENE; Roche) and cultured for a further 48 hours before processing for immunofluorescence analysis.

For the rescue experiments, siRNA-resistant expression constructs expressing AMOT p80, AMOT p130, AMOT p130ΔPPXY, AMOTL1 or AMOTL2 were co-transfected at t = 24 hr.

In summary, we are able to induce and functionally rescue loss of endogenous ATG16L1 expression using ATG16L1 RNAi-resistant expression constructs carrying no epitope tag.

Fcp1 expression was efficiently downregulated by the specific siRNAs, whereas the siRNA-resistant expression vector restored Fcp1 expression to about twofold over the levels of control cells.

BIRC3 and cREL siRNA-resistant expression constructs were designed by including silent mutations in the siRNA binding sites and synthesized by GeneArt (Life Technologies-Thermo Fisher, Carlsbad, CA, USA).

Cells were co-transfected with expression vectors for HIV-1ΔPTAP, ΔYP (lanes 1 7), FLAG-NEDD4L (lanes 3 7), and siRNA-resistant expression constructs for AMOT p80 (lane 5), HA-AMOT p130 (lane 6) or both (lane 7).

To verify the requirement for the kinase activity of Akt (a downstream target of IGF1R) in the mitogenic action of estradiol, we used shRNA strategy and shRNA-resistant expression vectors.

Cells were co-transfected with a control siRNA (lane 1) or with an siRNA that depleted AMOT p130 and AMOT p80 (lanes 2 5), and with expression vectors for HIV-1 (lanes 1 5), with siRNA-resistant expression constructs for AMOT p130 (lane 3), AMOTL1 (lane 4) or AMOTL2 (lane 5).