Sentence examples for resistant embryonic from inspiring English sources

Hygromycin resistant embryonic stem cell colonies were picked, expanded, and tested by PCR to identify clones in which the PGK-Hygromycin gene had correctly replaced exon 2 of the GPRC6A gene.

R1 (129Sv) embryonic stem (ES) cells were grown on a layer of G418 (Invitrogen) resistant embryonic feeder cells in a medium supplemented with leukemia inhibitory factor.

We confirmed homologous recombination in 11% of the neomycin-resistant R1 embryonic stem cells (Figure 4A, B).

A recently reported direct caspase activator 1541 has been shown to induce cell death in multidrug-resistant mouse embryonic fibroblasts (MEFs) deficient for both Bax and Bak.

Ssd also proved to be a potent cytotoxic agent in apoptosis-defective or apoptosis-resistant mouse embryonic fibroblast cells, which either lack caspases 3, 7 or 8 or had the Bax-Bak double knockout.

Transfected RhiPSCs were passaged onto puromycin-resistant DR4 mouse embryonic fibroblast (MEF) feeders (GlobalStem) on day 2, and 0.5 ug/ml of puromycin (Sigma-Aldrich) was added on day 3, and continued for 10 days thereafter, for selection of targeted colonies.

Thus, we sought to establish an efficient and quantitative reprogramming assay via cell fusion between mouse ESCs and G418-resistant (Rosa26) mouse embryonic fibroblasts (MEFs) that carry the Oct4-gfp transgene [20], [21].

In vitro, embryonic myoblasts are resistant to the differentiation inhibitory effects of transforming growth factor beta and phorbol esters.

Eventually this accumulation of marks results in a permanently silent state, one that is retained through the mammalian life cycle and is resistant to perturbations such as embryonic demethylation.

Most importantly, cyclin E knockout mouse embryonic fibroblasts were resistant to oncogenic transformation, confirming the crucial role played by cyclin E in tumorigenesis.

In eutherian mammals, the embryonic gonads are resistant to sex steroid effects although estrogen is required to maintain the postnatal ovary.