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Since the cell lines transfected with RNA from DEB025res and CsAres cultures proved also resistant to the drugs, although not to the full extent as the originally selected resistant cultures; it is concluded that resistance is at least in part associated with the viral genome, although cellular changes may also play a role as discussed below.
As previously reported, resistant cultures exhibited a high ERK activation correlated with BRAFi resistance, with an overall IC50 increase of 20- to 400-fold (Greger et al., 2012; Su et al., 2012; Figure S1A).
Efficient depletion of RECQ1 protein was observed in the whole cell extracts of puromycin resistant cultures that harbor RECQ1 shRNA plasmid as compared to the control (Fig. 4A).
LCKan plasmid DNA bearing the rescued pMOD700 transposon was prepared from resistant cultures with the High Pure Plasmid Isolation Kit (Roche).
The biggest share of resistant cultures was to the oxygen-contained biocides - 6.3% of 29 strains tested to this class.
In agreement with microarray data sets, RNF125 transcript and protein levels were markedly decreased in the two resistant cultures (A375R and Lu1205R) compared with parental cultures.
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Click here to view Creating their own space and identity is the next challenge for the generation of performers ready to admit to their sexuality in a notoriously resistant culture.
This has often led to a tension at the festival between those who consider it part of 'their' (at least passively) resistant culture – one which they've always expected to be free of charge – and those for whom it's a long weekend in rolling hills where you can watch the cover stars of the NME and Mojo in the same place, albeit for a large fee.
Sensitivity results were compared with a standard susceptible strain of H. pylori (NCTC # 12822) and the University of Virginia Center for Studies of Diseases Due to H. pylori metronidazole resistant culture strain #Cp 2124 and clarithromycin resistant strain #Cp 5535.
A clonal line of DEC-R parasites was subcloned from the resistant culture for analysis by DNA microarray and dose-response analysis confirmed a 90-fold increase in the IC50 compared to the decoquinate-sensitive parental strain (Supplementary Figure 1, panel b).
Nisin-producing strains in combination with other nisin resistant or tolerant cultures with desirable properties have been proposed as an alternative to the addition of nisin in commercial form.
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