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An acidic region of Rad52, encompassing residues 308 311 is required for interaction with RPA and for DNA damage resistance (Plate et al. 2008).
The repair capabilities of MSH2 missense mutations have been previously characterized using complementation assays in bacteria and resistance plate assays in yeast (Ellison, et al., 2001; Gammie, et al., 2007; Lützen, et al., 2008; Martinez and Kolodner, 2010).
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Purified product was cloned according to the StrataClone PCR Cloning Kit (Agilent Technologies, Santa Clara, CA, USA) and plated on ampicillin resistance plates.
Isolates with reduced sensitivity against oxacillin or erythromycin were further characterized by determining the minimal inhibitory concentration (MIC) of penicillin, erythromycin, ceftriaxone, and ciprofloxacin by using the E-test (AB Biodisk) on resistance plates (4.6 mm; SSI).
Transformants were selected with kanamycin-resistance plate.
The high resistance to plate coarsening exhibited by Ω plates at temperatures up to 200°C, is due to a prohibitively high barrier to ledge nucleation in the strong vacancy field normal to the broad face of the plate.
Increasing the cooling temperature decreased the thermal resistance of plate OHPs.
The resistance of plate girders subjected to patch loading has been studied thoroughly for the case of homogenous girders.
Aliquots of 2 ml cultures were treated with respective antibiotics to determine phenotypic antibiotic resistance by plate count method (cfu counting).
Bacteria harvested from the spleen on day 4 post-infection were monitored for loss of ampicillin resistance by plating initially on HIA, then patching colonies onto plates with and without ampicllin.
In this paper, Ansys Fluent was used to simulate the flow field of an existing high-resistance grate plate.
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