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Tetrads were pulled, and haploids containing both HIS3 (TAP) and NAT-resistance (deletion) markers were selected.
In Saccharomyces cerevisiae [66], an AP-1 transcription factor (YAP-1) is involved in the response to oxidative stress and metal resistance; indeed deletion mutants in the gene encoding this transcription factor are hypersensitive to the presence of cadmium in the medium.
To assess the effects of lactogen resistance (PRLR deletion) on thermogenesis, we subjected newborn mice to a cold challenge and calculated their survival rates during the 400-min period, since alternative methods such as measurement of rectal temperature or indirect calorimetry could not be performed on newborn animals.
Transformants were screened for hygromycin resistance and deletion of Hep1 was identified by PCR.
Trx-1 regulates aging and stress resistance; its deletion shortens adult lifespan and increases the sensitivity to paraquat-induced oxidative stress.
The relationship between the toxicity of yeast cells and the resistance of deletion strains of the MVB pathway to nickel compounds is not clear.
A metal-specific response identified in both our study and that of Ruotolo et al. is the resistance of deletion strains of components of the MVB pathway (ESCRT complexes) to nickel.
To determine the role of GltK and GspA in bile salt resistance, markerless deletion mutants in gltK and gspA were constructed in E. faecium E1162, and the mutants were complemented in trans.
Surprisingly, only 4% (12/287) of genes were identified in both the overexpression-resistance and deletion-sensitivity assays.
To further study the degree of sensitivity or resistance of each deletion strain in our phenotypic screen, the IC50 for each deletion strain was analyzed in a secondary validation screen and the target strains were arranged based on their degree of sensitivity to NiSO4.
Based on homology with the class IIb HDAC6 complex in mammalian cells, we predicted that Hda1 was a key KDAC for azole resistance and that deletion of HDA1 from an erg3 mutant would abrogate azole resistance, as with trichostatin A. However, deletion of HDA1 had no impact on azole resistance of an erg3 mutant.
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