Sentence examples for resin manual from inspiring English sources
Finally, the loaded resin was incubated with 4 CV of elution buffer according to the GenScript resin manual for 15 min at 37°C and eluted by centrifugation in an empty Mini Bio-Spin column at 1000 g.
The supernatant was collected, dialysed with a Millipore 10 kDa cut off membrane device at 4 °C to remove ions and salts and subjected to a Ni2+-affinity chromatography column as described in the Ni NTA resin manual from TransGen Biotechnology (China) to remove other proteins and small peptides.
Current development of composite components made by Resin Transfer Moulding (RTM) requires numerous manual iteration steps to find the optimal design in conjunction with optimal process control.
The fusion protein was affinity purified in urea using Ni-NTA resin as described in User's Manual (Amersham Pharmacia Biotech), and then used to produce mouse monoclonal antibodies.
Recombinant proteins were purified using glutathion Sepharose affinity resin following the pGEX instruction's manual.
In general, this type of foundry applies manual moulding techniques with resin-bonded sand moulds.
The supernatant was filtered through a 0.45-μm filter and the recombinant TreS was purified through a Ni NTA affinity chromatography column according to the manufacturer's purification protocol manual (Novagen, Ni NTA HisBind Resins).
The NTA resin was prepared by following the manufacturer's manual.
Because of the perceived tendency of resins to discolor, technical manuals instructed commercial painters to make white and light-colored house paints using raw linseed oil and/or stand oil with very small quantities of resins, if any, whereas the addition of resins in large quantities was strongly recommended for darker colored enamel paints.
The main disadvantages of this method are: (i) the use of resins (organic compounds) which are hazardous for the manual worker, and (ii) the low permeability of this type of organic materials.
Manual synthesis included the following steps: (i) resin washing with DMF (5 × 30 s); (ii) Fmoc removal with 20% piperidine/DMF (1 × 1 min + 2 × 7 min); (iii) washing with DMF (5 × 30 s); (iv) washing with DMF (5 × 30 s) and CH2Cl2 5 × 30 s); (v) Kaiser's test (with a peptide-resin sample); (vi) DMF washing (5 × 30 s).
