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A maximum load resin capacity of 100 g Ni/kg was obtained in these tests.
Once a certain minimum resin ligand density is supplied, additional ligand is not beneficial in terms of resin capacity.
Open image in new window Figure 3 World thermoplastic resin capacity, 2008 (adapted from [[10]]).
The amount of resin to be employed was calculated considering wet resin capacity and total metal content.
The observed destabilization may reflect properties specific to NTS1: In contrast to NTS1, purification of an adenosine A2a receptor fusion protein by antagonist xanthine amine congener affinity chromatography with an estimated resin capacity of 14 mM did not lead to substantial amounts of A2a receptor protein in the antagonist column flow-through [23].
Binding assays can determine resin capacity from the mobile phase, but do not reveal the underlying causes of Protein A ligand degradation.
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Resin capacities were calculated according to breakthrough points in a fixed bed flow system which is the first step in designing of solid phase extraction (SPE) module for arsenic speciation separation and determination.
While it was not surprising that increasing the peptide-to-resin capacity resulted in higher abundances of phosphorylated peptides, it was observed that the addition of 50 or 100 mg/mL of MassPrep Enhancer reduced the summed intensity of phosphorylated peptides by 7% or 25%, respectively, at a peptide-to-resin capacity ratio of 2 1.
The global specificity for all phosphopeptides varied from 6% at a peptide-to-resin capacity ratios of 0.1 1 and 0 mg/mL MassPrep Enhancer up to 80% at a peptide-to-resin capacity ratios of 2 1 and 300 mg/mL MassPrep Enhancer.
Surprisingly, an increase in global phosphopeptide enrichment specificity by as much as 64% was observed by simply increasing the ratio of peptide-to-resin capacity from 0.1 1 to 2 1.
The strategy utilizes a TiO2 enrichment protocol in which the selectivity and recovery of phosphopeptides were optimized by assessing a twenty-point condition matrix of binding modifier concentrations and peptide-to-resin capacity ratios.
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