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1-Methyl and 1-phenyl-ortho-carborane functionalized polystyrenes (1a, 1b) were synthesized by the reactions of pertinent carborane lithium salts and Merrifield's peptide resin (1%), in refluxing tetrahydrofuran (THF) solvent in 90 and 85% yield, respectively.
where C is the metal concentration in HNO3 solution measured by ICP-MS, F is the dilution factor (5), V acid is the volume of HNO3 added to the resin (1 mL), V gel is the volume of resin gel (0.15 mL), f e is the elution factor (0.8).
The amino acid derivative 4 (Figure 10) was cleaved from the resin (1 g) by incubating with TFA DCM (1∶1) for 2 hours.
Anti-E-tag mAb (1 mg, Phadia) was previously crosslinked to protein G-Sepharose resin (1 ml, Sigma) with dimethyl pimelimidate dihydrochloride (DMP; Sigma).
The lysate was clarified by centrifugation at 12800×g for 15 min, then loaded directly onto a column of High-Affinity GST Resin (1 mL bed volume; Genscript) that had been equilibrate with 20 bed volumes ice-cold PBS containing 1 mM PMSF.
was coupled to deprotected Rink Amide Resin (1 eq).
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The object is a pillar of resin, 10 feet in height, four feet in diameter.
A mixture of acetone and resin (1 1) was used for 1 h resin infiltration, which was followed by overnight infiltration with 100%% resin.
The average exchange capacity of resin discharged from the bed is 0.57 mmol · (g dry resin)−1, which is close to the saturated capacity of 0.65 mmol · g−1.
Subsequent oxidation of these radicals to the corresponding cations in the presence of silver hexafluoroantimonate (AgSbF6) leads to the simultaneous formation of silver nanoparticles and initiation of cationic polymerization of epoxy resin, 1,3-bis 3,4-epoxycyclohexylethyl tetramethyldisiloxane (EPOX) present in the formulation.
Cation exchange resin (001 × 7) and anion exchange resin (201 × 7) were purchased from Bohong Company (Tianjin, China).
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