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Radiation damage can also be responsible for decarboxylation of aspartate and glutamate residues, loss of the hydroxyl group from tyrosines and the methylthio group from methionines, as discussed by Matsui et al [30].
The reversibility of unfolding was studied using various parameters including the exposure of tryptophan residues, loss of secondary structure, and the exposure of hydrophobic surface.
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Among the isolates, highest residue loss was realized with A. flavus RPW 1/3 (31%) followed by A. terreus RPW 1/6 (29%) > Alternaria alternata RZWM 3/2 (26%) > P.
It is still unclear which patterns and processes affect cysteine residue loss in PI-II.
With the knowledge of the unique fragmentations leading to internal residue losses, the misidentification of fragments and sequences of peptides may be avoided.
Neither singly nor triply charged peptides are observed with abundance, although low levels of peptides in these charge states can be identified by characteristic amino acid residue losses in MS/MS spectra.
cR8 forms extensive hydrogen bonding with SPSB2 residues, but loss of an intramolecular hydrogen bond that is present in SPSB2-bound iNOS peptide may destabilize the bound conformation of cR8 and lead to a gentle reduction in SPSB2 binding affinity.
Therefore, even though it is difficult to unequivocally decide whether bulky mutant residues cause loss of function because they change a critical residue or because they partial unfold the protein, our results suggests that this region is indeed a very critical one for proper 2-HBP-mediated activation of HbpR and that its most essential residues are Thr52, Ile180, Glu184 and Cys187.
However, proline scanning of all residues revealed loss of zymosan interaction with TLR2-ECD.
2G12-IgM tolerates 17 germline residues without loss of antigen binding.
This results in a frame shift after 51/281 amino acid residues and loss of the entire bHLH domain, implying that this is a null allele.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com