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Exact(5)
The dried residues dissolved in EtOAc, and partitioned with water.
The solvent was evaporated and the residues dissolved in MeOH and analysed as described above.
Hexane extracts were dried using anhydrous magnesium sulphate and evaporated under reduced pressure and residues dissolved in 1.5 mL of hexane.
The organic phases were combined, evaporated under a stream of argon and residues dissolved in chloroform:methanol (2 1, 350 μl) before storing at −20 °C for analysis by GCMS.
Regardless of the extraction conditions or used acid, in the chromatograms of the extracts peak intensities were significantly lower when they were evaporated and residues dissolved in methanol, than in the chromatograms of the solutions obtained just after extraction (Fig. 3).
Similar(55)
The mixture was again evaporated to dryness and the residue dissolved with 0.5 mol L-1 HNO3.
The solvent was removed under reduced pressure, and the resulting residue dissolved in 1 mL MeCN.
The solvent was evaporated, and the residue dissolved in chloroform the solution washed with sodium bicarbonate solution.
After freezing, the ether phase was collected, dried under nitrogen and the residue dissolved in the appropriate assaybuffers.
The solvent was removed under reduced pressure, and the residue dissolved in Et2O (25 mL).
The organic phase was evaporated to dryness in a stream of N2 and the residue dissolved in 1 ml toluene.
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