Using synthetic peptides, three different proteolytic activities were measured to detect proteasome activities: (1) chymotrypsin-like (after hydrophobic residue), (2) trypsin-like (after basic residues), or (3) PGPH (after basic residues) activities [13].
Corresponding to the function of the different subunits of the catalytic core, the activities of the 20S proteasome include (1) chymotrypsin-like (after hydrophobic residues), (2) trypsin-like (after basic residues), or (3) peptidyl-glutamyl preferring hydrolytic (PGPH, after basic residues) activities [12], [13].
This specific proteolytic activity of immunoproteasomes relates to the particular function of immunoproteasomes for the generation of MHC class I epitopes: reduced caspase-like (cleavage after acidic residues) and enhanced chymotrypsin-like (cleavage after hydrophobic residues) activities of immunoproteasomes results in the preferential generation of peptides with hydrophobic C-termini.
For slow, reversible inhibition with fractional residue activity, the kinetic model of the enzyme reacting with the substrate and the inhibitor can be written as Scheme 1[30], where S, P, Hg and E respectively denote substrate, product, inhibitor (Hg2+) and enzyme.
At alkaline pH, immobilized lipases were more stable than free lipase (residue activity more than 63.4% at pH 11 for 3 h).
Bounding ability of carrier which is determined by residue activity of enzyme solution is one of the most important index immobilizations of enzyme.
At alkaline pH, immobilized lipases were more stable than free lipase (more than 60% residue activity at pH 11 for 3 h).
The weak potency of tarenflurbil can be attributable to low CNS penetration as shown in phase 1 trial, and on the other hand, NSAID residue activity curbed A β clearance mechanism mediated by microglia [ 69].
The activity of 68Ge in 68Ga-DOTATATE residues with activities in the range between 10 and 30 MBq 68Ga was in nearly all cases below 10 Bq of 68Ge.
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Tryptophanamides with aliphatic side chains were inactive whereas basic residues restored activity.
