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Using the remaining residues, a second prediction was performed.
To reduce pectin and tannin residues, a second chloroform wash was carried out.
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By adding methanol (100 μL) to the residue, a second methanol extraction was carried out in the same fashion.
In addition to the canonical MBOAT homology domain, we also identified residues in a second region (residues 196– 234) that are highly conserved in family members that transfer fatty acids onto protein substrates.
To demonstrate that the general net positive charge of a transit peptide but not a specific aa residue at the N-terminus of plastid-destined precursor is crucial for import, we replaced the glutamate residues with arginine residues in a second round of mutagenesis.
Although 56 residues from each chain contribute to a dimer interface as large as 2200 Å2 in wild-type TetR, the substitution of only three residues in one chain and two residues in a second chain sufficed for generating specificity in a T-AAB heterodimer variant.
Two more positively selected residues form a second cluster falling in or near the site 3 IL13Rα1-binding epitope used by the Type II IL4 receptor.
The 5′-nucleotide-binding site shares common residues with a second, adjacent ligand-binding site, suggesting a mechanism for the cooperative binding of ligands to the MID domain of eukaryotic AGOs.
However, combined R97/Y105 mutation reduced NF-κB to the level of the death domain deletion mutant which identifies these residues as a second crucial binding site for the NF-κB activating activity of IRAK-M in 293T cells.
Despite the low abundance of non membrane-bound, helical α-synuclein molecules, these species may initially interact with membranes via conformational selection and trigger additional helical transitions of more C-terminal α-synuclein residues in a second induced fit step.
In addition to the conservative disulfide bond tightening the beta-barel to the C-terminal tail [ 28] this extra two cystein residues form a second disulfide bridge characteristic for this group of lipocalins [ 29].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com