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We show that choosing the primary rotation angle θ1,3 can define the flanking rotations θ1,2 and θ2,3 via kinematics and minimization, and is hence sufficient to parameterize a backrub for a given residue, resulting in a 1 degree-of-freedom per residue design problem.
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The most potent analog, Oxpholipin-11D (OxP-11D), contained D-amino acids exclusively and was identical to the 14-residue design template except that diphenylalanine replaced cysteine-3.
Several Gly residues designed as linker.
The resulting 17-residue designed sequence is 41% identical to the relevant regions in ROP.
Far-UV circular dichroism was used to characterise the solution conformation of a series of peptides, containing either four or six histidine residues, designed to respond at differing pH while a novel application of near-UV circular dichroism was used to determine the binding affinities of the peptides for both DNA and siRNA.
The β-turn (also known as reverse turn) is a supersecondary structure originally described by Venkatachalam [ 43] that consists of 4 residues designed as "i", "i+1", "i+2", "i+3".
Indeed, single point mutations of these residues, designed to disturb these structural features, reduced EPCR binding affinity by up to 100-fold.
"Luck is the residue of design," Branch Rickey liked to say, and his design for baseball turned out to be a design for America.
Or, as Rickey himself put it, "Luck is the residue of design".
[cartoon id="A17721"] Yet luck is the residue of design and devotion.
In the words of that profound thinker Branch Rickey, "Luck is the residue of design".
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CEO of Professional Science Editing for Scientists @ prosciediting.com