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Exact(4)
LGE imaging offers the ability to assess the transmural extent of residual viability in patients after myocardial infarction.
This effect was dose dependent, with the greatest difference observed at 16 U/L of XO (in the presence of 0.5 mM HX): following overnight treatment, the residual viability at this dose – the second largest tested – was 84.6±12.0% for RR cells but only 14.1±3.9% for RS cells.
Figure 2C (upper panel) shows residual viability at E T = 5 1.
After drug treatment and a recovery period, residual viability was measured by [3H] leucine incorporation followed by scintillation counting or by [35S] methionine incorporation and autofluorography in situ.
Similar(56)
The curves for unarmed ATC and CD20Bi-armed are essentially identical and show residual viabilities fluctuating around 100%.
The mean residual viabilities (and SEM) for 4 ATC donors are shown when these cells were used as targets, over E T ratios ranging from 1.5 1 to 12.5 1.
Percentage of residual cell viability was determined as [1−(OD of treated cells/OD of control cells)]×100.
Cells were trypsinized, resuspended in phosphate buffer saline (PBS), incubated with 0.4% trypan blue (prepared in PBS) for 8 min and counted (at least 600) for calculation of percentage of residual cell viability, as previously described [26].
Residual cell viability was assessed using the MTT assay.
When ATC were armed with EGFRBiAb or HER2BiAb residual target viability clearly was lower.
After 48 h, the cells were exposed for 2 h to E. coli DH5α (~100 bacteria per epithelial cell), and residual bacterial viability was assessed by plating serial dilutions of the conditioned media on LB agar.
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